International Standard
ISO/TS 13136:2012
Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
Reference number
ISO/TS 13136:2012
Edición 1
2012-11
International Standard
Vista previa
ISO/TS 13136:2012
53328
No disponible en español
Publicado (Edición 1, 2012)
Esta norma se revisó y confirmó por última vez en 2019. Por lo tanto, esta versión es la actual.

ISO/TS 13136:2012

ISO/TS 13136:2012
53328
Idioma
Formato
CHF 129
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Resumen

ISO/TR 13136:2012 describes the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.

In any case, when one or both of the stx genes is/are detected, the isolation of the strain is attempted.

The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).

The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.

ISO/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.

Informaciones generales

  •  : Publicado
     : 2012-11
    : Norma Internacional para revisar [90.92]
  •  : 1
     : 22
  • ISO/TC 34/SC 9
    07.100.30 
  • RSS actualizaciones

Próxima versión en desarrollo

Draft International Standard
ISO/DIS 13136-1
Microbiology of the food chain — Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) — Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC)
Reference number
ISO/DIS 13136-1
Edición 1
Draft
International Standard

ISO/DIS 13136-1

Microbiology of the food chain — Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC)

Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC)

ISO/DIS 13136-1

ISO/DIS 13136-1
84813
Idioma
Formato
CHF 63
Convertir Franco suizo (CHF) a tu moneda

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