Resumen
The method consists in extracting of the caffeine from a test portion, in an ammoniacal medium, successive purifying, with diethyl ether, on two chromatographic columns, the first in an alkaline medium, the second in an acid medium, followed by elution by chloroform. Spectrometric measurement of the eluate at the wavelength of maximum absorbance (in the ultraviolet region). A first figure shows examples of chromatographic columns. A second figure gives an example of spectrometric measurement.
Informaciones generales
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Estado: RetiradaFecha de publicación: 1983-06Etapa: Retirada de la Norma Internacional [95.99]
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Edición: 1Número de páginas: 6
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Comité Técnico :ISO/TC 34/SC 15ICS :67.140.20
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