Reference number
ISO 4052:1983
© ISO 2024
ISO 4052:1983
Coffee — Determination of caffeine content (Reference method)
Edition 1
1983-06
Withdrawn
Withdrawn (Edition 1, 1983)

Abstract

The method consists in extracting of the caffeine from a test portion, in an ammoniacal medium, successive purifying, with diethyl ether, on two chromatographic columns, the first in an alkaline medium, the second in an acid medium, followed by elution by chloroform. Spectrometric measurement of the eluate at the wavelength of maximum absorbance (in the ultraviolet region). A first figure shows examples of chromatographic columns. A second figure gives an example of spectrometric measurement.

General information

  • Status
     : Withdrawn
    Publication date
     : 1983-06
    Stage
    : Withdrawal of International Standard [95.99]
  • Edition
     : 1
    Number of pages
     : 6
  • Technical Committee :
    ISO/TC 34/SC 15
    ICS :
    67.140.20 
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