ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.
Status: PublishedPublication date: 2016-08
Edition: 1Number of pages: 21
Technical Committee: ISO/TC 229 Nanotechnologies
Buy this standard
|std 1 118|
|std 2 118||Paper|